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霍乱单克隆抗体重氮基乳胶试剂(DPRC)对霍乱弧菌的实验室检测获得较理想的结果。在现场应用中,对509份霍乱病人、疑似病人及接触者粪便标本检测,用常规培养分离法检出阳性54份,其过程平均需时22h以上;而DPRC法检出55份,平均需时为9.4h,两法结果完全相符(DPRC法多检出的1个阳性标本经再培养分离亦证实为阳性)。本研究结果表明,DPRC特异性强、敏感性高、稳定性好,使用简便而经济,尤其是具有快速诊断霍乱及普遍运用于各层次医疗卫生单位的优点,这对于霍乱防治的实际工作具有重要意义。
Cholera monoclonal antibody diazo latex reagent (DPRC) laboratory tests Vibrio cholerae to obtain better results. In the field application, stool specimens from 509 cholera patients, suspected patients and contacts were detected, and 54 positive samples were detected by conventional culture separation method, taking an average of 22 hours or more; DPRC detected 55 samples on average 9.4h, the two results are completely consistent (more than one DPRC France detected positive samples were isolated from culture also confirmed as positive). The results of this study show that DPRC has the advantages of high specificity, high sensitivity, good stability, simple and economical use, especially the advantages of rapid diagnosis of cholera and universally applicable medical and health units at all levels, which is important for the practical work of cholera prevention and control significance.