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目的:研究B7-1基因在急性白血病(AL)患者中的表达及临床意义。方法:应用逆转录聚合酶链反应(RT-PCR)检测64例AL患者B7-1mRNA的表达水平,14例健康人为正常对照,K562、NB4细胞株为阳性对照。结果:AL患者B7-1基因的表达率与对照组没有差异,但半定量后的表达水平明显较正常低,且初发和缓解患者表达量差异无显著性。结论:B7-1基因表达的紊乱可能在AL的发病中起着重要作用,并且可能与AL复发和预后相关。
Objective: To study the expression of B7-1 gene in patients with acute leukemia (AL) and its clinical significance. Methods: The expression of B7-1 mRNA in 64 AL patients was detected by reverse transcription-polymerase chain reaction (RT-PCR). 14 healthy individuals were normal controls and K562 and NB4 cell lines were positive control. Results: The expression of B7-1 gene in AL patients was not different from that in control group. However, the expression level of B7-1 in AL patients was significantly lower than that in normal controls. There was no significant difference in the expression of B7-1 between AL patients and controls. Conclusion: The disturbance of B7-1 gene expression may play an important role in the pathogenesis of AL, and may be related to AL recurrence and prognosis.