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以乌饭树单芽茎段和幼嫩叶片为试材,通过比较不同消毒方法、取材部位、茎段放置方式以及激素对乌饭树初代培养的作用,研究了不同因素对其初代培养中芽诱导效果的影响。结果表明:75%酒精30s+0.1%升汞6min消毒效果最佳,污染率为0,褐化率较低,萌芽率较高,展叶天数较早,且消毒后的外植体生长状况最佳;乌饭树单芽茎段较其叶片用作试验材料较好;放置方式以茎段横置于培养基中较佳;相较其它激素,ZT对乌饭树的诱导效果好,而各浓度的ZT中,以4mg/L效果最佳。
The results showed that the effect of different factors on the buds of their primary culture buds was studied by comparing the different methods of disinfection, Effect of induction. The results showed that: 75% alcohol 30s + 0.1% mercuric chloride 6min the best disinfection effect, the pollution rate is 0, the browning rate is low, the germination rate is higher, the number of leaf days is earlier, and the growth of the explants after disinfection Compared with the other hormones, the induction effect of ZT was better than the other hormones, while the effects of ZT Concentration of ZT, the best 4mg / L.