目的 :探讨 Castleman′s病与滤泡性淋巴瘤的鉴别诊断方法。方法 :应用免疫组化方法对 19例Castlem an′s病与 2 2例滤泡型淋巴瘤进行 bcl- 2肿瘤基因蛋白和κ、λ表达测定。结果 :19例 Castleman′s病不论何种亚型 17例生发中心细胞 bcl- 2阴性 ,κ、λ呈多克隆表达。 2 2例滤泡性淋巴瘤中 19例 bcl- 2呈阳性 ,3例 bcl- 2阴性 ,κ、λ呈单克隆表达。表达 bcl- 2、κ和λ,X检验 Castleman′s病与滤泡性淋巴瘤两者在 bcl- 2、κ和λ表达方面有显著性差异 (P<0 .0 1)。结论 :bcl- 2结合κ、λ对 Castlem an′s病与滤泡性淋巴瘤具有一定的鉴别诊断意义 Objective: To explore the differential diagnosis of Castleman’s disease and follicular lymphoma. METHODS: Immunohistochemistry was used to detect the expression of bcl-2 oncogene protein and κ and λ in 19 cases of Castele an’s disease and 22 cases of follicular lymphoma. RESULTS: Of the 19 cases of Castleman’s disease, no matter which subtype, 17 cases of germinal center cells were bcl-2 negative, and kappa and lambda were expressed in multiple clones. Of the 22 cases of follicular lymphoma, 19 cases were positive for bcl-2, 3 cases were negative for bcl-2, and kappa and lambda were expressed as single clones. Expression of bcl-2, κ, and λ, X test Castleman′s disease and follicular lymphoma have significant differences in bcl-2, κ, and λ expression (P<0.01). Conclusion : Bcl-2 binding to κ and λ has a certain value in the differential diagnosis of Castlem an’s disease and follicular lymphoma