论文部分内容阅读
NPR1(Non-expresser of pathogenesis related genes 1)是水杨酸(Salicilic acid,SA)介导的系统获得抗性(Sys-temic aquired resistance,SAR)的关键调控因子,在水稻中过量表达拟南芥NPR1和水稻NH1/OsNPR1(NPR1 homo-logue 1)(NPR1的同源物)可增强抗病性。水稻基因组中还有4个NPR1旁系同源物(Paralog),为NH1的家族蛋白。本试验利用双分子荧光互补技术(Bimolecular Fluorescence Complementation,BiFC,or split YFP)研究了水稻NH1家族蛋白之间在活体内的相互作用,发现除NH2外,NH1、NH3、NH4和NH5都会和自身的蛋白互作,产生荧光信号,并且它们还会和家族成员的其他蛋白相互结合产生荧光信号。
NPR1 (Non-expresser of pathogenesis related genes 1) is a key regulator of Salicilic acid (SA) -mediated Sys-temic aquired resistance (SAR), overexpressing Arabidopsis thaliana NPR1 and rice NH1 / OsNPR1 (NPR1 homo-logue 1), a homologue of NPR1, enhance disease resistance. There are also four NPR1 paralogs in rice genome, which are the family proteins of NH1. In this study, Bimolecular Fluorescence Complementation (BiFC, or split YFP) was used to study the in vivo interactions between rice NH1 family proteins. It was found that NH1, NH3, NH4 and NH5, except for NH2, The proteins interact to produce fluorescent signals, and they also interact with other proteins in family members to produce fluorescent signals.