论文部分内容阅读
目的 :研究 HPV与喉上皮性肿瘤的关系。方法 :采用一对 HPV L1区通用引物及 6、1 1、1 6、1 8型特异 E6区引物多聚酶链反应 ,对 1 2 8例喉癌及喉上皮增生性病变样本进行 HPV检测。结果 :2 4例检出HPV,1 6例为 HPV6型 ,4例 HPV 1 1型 ,1例 HPV 1 8型 ,2例未定型。 60例喉癌中 5例阳性 ,其中 3例 6、1 1型均为整合状态 ,另 2例为未定型。结论 :大部分喉癌和上皮增生性病变与 HPV感染无关 ;良性型 HPV可整合于宿主细胞核 ;对少数 HPV阳性患者 ,除采用手术、化疗及放疗外 ,还需抗病毒治疗。
Objective: To study the relationship between HPV and laryngeal epithelial tumors. Methods: A total of 128 samples of laryngeal squamous cell carcinoma and laryngeal epithelial hyperplastic lesions were detected by using a pair of HPV L1 universal primers and 6,1 1, 11, 18 specific E6 primers. Results: 24 cases were detected HPV, 16 cases were HPV6 type, 4 cases of HPV type 1 1, 1 case of HPV type 1, 2 cases were not stereotyped. Of the 60 laryngeal carcinomas, 5 were positive, of which 3 were type 6 and 1 1, and the other 2 were undetermined. Conclusion: Most of the laryngeal carcinoma and epithelial hyperplastic lesions have nothing to do with HPV infection. The benign HPV can be integrated into the nucleus of host cells. For a few HPV positive patients, antiviral therapy is required besides surgery, chemotherapy and radiotherapy.