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目的:研究鼻咽癌原发灶和自身颈部淋巴结转移癌组织中上皮型钙黏附蛋白(E-Cadherin)和β-链接素(β-catenin)表达水平的差异性。方法:采用免疫组化En Vision法检测22例鼻咽癌患者原发灶和自身颈部淋巴结转移癌组织中E-Cadherin和β-catenin基因产物的表达水平。结果:(1)22例鼻咽癌患者淋巴结转移癌组织中的E-Cadherin阳性表达率(40.9%)显著低于22例原发灶组织中的表达水平(63.6%)(P<0.05);(2)β-catenin在原发灶和淋巴结转移癌组织中均有较高的表达(86.4%,90.9%),未显示有差异性(P>0.05);(3)原发灶与颈部淋巴结转移癌组织中癌细胞表达的E-Cadherin呈正相关(r=0.676,P<0.01);转移癌组织中E-Cadherin与β-catenin呈正相关(r=0.507,P<0.05)。结论:(1)在鼻咽癌组织中,导致癌细胞侵袭转移的一个重要因素可能是E-Cadherin的表达下调。(2)因某些原因积聚于细胞内的β-catenin可能在协同促进鼻咽癌细胞侵袭转移过程中起作用。(3)在肿瘤侵袭转移的过程中,E-Cadherin与β-catenin之间可能通过某些细胞信号通路连接进而相互调节,相互影响。
Objective: To study the difference of the expression of E-Cadherin and β-catenin in primary nasopharyngeal carcinoma and its own cervical lymph node metastasis. Methods: The expressions of E-Cadherin and β-catenin in primary cervical cancer tissues and cervical lymph node metastases tissues of 22 NPC patients were detected by immunohistochemistry En Vision method. Results: (1) The positive expression rate of E-Cadherin (40.9%) in 22 cases of NPC with lymph node metastasis was significantly lower than that in 22 cases (63.6%) (P <0.05). (2) The expression of β-catenin in primary tumor and lymph node metastasis was high (86.4%, 90.9%), which showed no difference (P> 0.05); (3) There was a positive correlation between E-Cadherin expression and cancer cells in lymph node metastasis (r = 0.676, P <0.01). There was a positive correlation between E-Cadherin and β-catenin in metastatic cancer tissues (r = 0.507, P <0.05). Conclusion: (1) An important factor in the invasion and metastasis of nasopharyngeal carcinoma may be the downregulation of E-Cadherin. (2) β-catenin accumulated in the cells for some reason may play a role in synergistically promoting the invasion and metastasis of nasopharyngeal carcinoma cells. (3) During the process of tumor invasion and metastasis, E-Cadherin and β-catenin may regulate and interact with each other through certain cellular signaling pathways.