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丝裂原活化蛋白激酶(M APK)是生物体内信号转导的重要组分,与生长、发育和逆境胁迫反应密切相关.为了研究草坪草对非生物逆境胁迫反应的分子机理,利用同源基因克隆法从4℃低温诱导的草坪草高羊茅(F estu-ca arund inacea Schreb.)幼苗cDNA文库中分离得到一个M APK的cDNA即F aMAPK 1,F aMAPK 1编码369个氨基酸残基的蛋白激酶,该蛋白激酶具有TEY的磷酸化基序.据推测的氨基酸序列的BLA ST同源性分析表明,F aM APK 1蛋白与水稻O sM APK 4蛋白的一致性为91.1%.N orthern杂交检测F aMAPK 1基因对逆境胁迫反应的结果表明冷(4℃)处理对根中F aMAPK 1基因的表达没有明显影响,但诱导叶中F aMAPK 1上调表达.而且低温(4℃)、高盐(250 mm o l/L N aC l)、干旱和100μm o l/L ABA都诱导叶中F aMAPK 1上调表达,表明F aM APK 1蛋白可能在高羊茅对非生物逆境胁迫的反应中起重要作用.
Mitogen-activated protein kinase (M-APK) is an important component of signal transduction in vivo and is closely related to the growth, development and stress response.In order to study the molecular mechanism of turfgrass response to abiotic stresses, Cloning method was used to isolate a M APK cDNA, F aMAPK 1, from a cDNA library of F estu-ca arund inacea Schreb. Induced by low temperature in 4 ℃. F aMAPK 1 encodes a protein of 369 amino acid residues Kinase with a phosphorylated motif of TEY.The BLA ST homology analysis of the deduced amino acid sequence showed that the identity of F aM APK 1 protein to rice O sM APK 4 protein was 91.1% .N orthern hybridization assay The results of F aMAPK 1 gene response to stress indicated that cold (4 ℃) treatment had no significant effect on the expression of F aMAPK 1 gene, but F aMAPK 1 was up-regulated in the induced leaves. Moreover, low temperature (4 ℃) 250 mm ol / LN aC l), drought and 100 μm ol / L ABA induced up-regulation of F aMAPK 1 in leaves, indicating that F aM APK 1 protein may play an important role in the response of A. fescue to abiotic stress.