AIM:To study the changes of quantitative expression,adheringactivity and genomic density polymorphism of complementtypes in erythrocytes(CR1)of patients with gallbladdercarcinoma and the related clinical significance.METHODS:Polyrnerase chain reaction(PCR),HindⅢ restrictionenzyme digestion,quantitative assay of CR1 and adheringactivity assay of CR1 in erythrocytes were used.RESULTS:The number and adhering activity of CR1 in patientswith gallbladder carcinoma(0.738±0.23,45.9±5.7)weresignificantly lower than those in chronic cholecystitis andcholecystolithiasis(1.078±0.21,55.1±5.9)and healthy controls(1.252±0.31,64.2±7.4)(P<0.01).The number and adheringactivity of CR1 in patients with chronic cholecystitis andcholecystolithiasis(1.078±0.21,55.1±5.9)were significantlylower than those in healthy controls(1.252±0.31,64.2±7.4)(P<0.05).There was a positive correlation between quantitativeexpression and adhering activity of CR1(r=0.79,P<0.01).Compared with those on preoperative day(0.738±0.23,45.4±4.9),the number and adhering activity of CR1 in patientswith gallbladder carcinoma decreased greatly on the thirdpostoperative day(0.310±0.25,31.8±5.1)(P<0.01),andon the first postoperative week(0.480±0.25,38.9±5.2)(P<0.01),but they were increased slightly than those onthe preoperative day(P>0.05).The number and adheringactivity of CR1 recovered in the second postoperative week(0.740±0.24,46.8±5.9)(P<0.01)and increased greatly in thethird postoperative week(0.858±0.35,52.7±5.8)(P<0.01)in comparison with those on the preoperative day and inthe first postoperative week.The number and adheringactivity of CR1 of gallbladder carcinoma patients withinfiltrating,adjacent lymphogenous and distant organmetastases were significantly lower than those ofgallbladder carcinoma patients without them(P<0.01).Nodifference was observed between the patients withgallbladder carcinoma and healthy individuals in the spotmutation rate of CR1 density gene(x~2=0.521,P>0.05).The distribution of expression was 67.8% in high expressiongenomic type,24.8% in moderate expression genomic type,and 7.4% in low expression genomic type.The number andadhering activity of CR1 high expression genomic type gallbladder carcinomas(0.749±0.22,42.1±6.2)weresignificantly lower than those of healthy individuals(1.240±0.29,63.9±7.2),and were also significantly lower than thoseof healthy individuals(0.921±0.23,54.8±7.1),but nodifference was observed between the number and adheringactivity of CR1 lower expression genomic type gallbladdercarcinomas(0.582±0.18,44.3±5.5)and those of healthyindividuals(0.610±0.20,45.8±5.7)(P>0.05).CONCLUSION:Defective expression of CR1 in gallbladdercarcinoma is mostly acquired through central peripheralmechanisms,The changes in CR1 quantitative expressionand adhering activity are consanguineously related to thedevelopment and metastasis in gallbladder carcinoma. AIM: To study the changes of quantitative expression, adhering activity and genomic density polymorphism of complement type of erythrocytes (CR1) of patients with gallbladder carcinoma and related related significance. METHODS: Polyrnerase chain reaction (PCR), HindIII restriction enzyme digestion, quantitative assay of CR1 and The adhering activity assay of CR1 in erythrocytes were used .RESULTS: The number and adhering activity of CR1 in patients with gallbladder carcinoma (0.738 ± 0.23, 45.9 ± 5.7) weresignificantly lower than those in chronic cholecystitis andcholecystolithiasis (1.078 ± 0.21, 55.1 ± 5.9) and healthy controls (1.252 ± 0.31, 64.2 ± 7.4) (P <0.01). The number and adhering activity of CR1 in patients with chronic cholecystitis and cholecystolithiasis (1.078 ± 0.21, 55.1 ± 5.9) were significantlylower than those in healthy controls (1.252 ± 0.31, ± 7.4) (P <0.05). There was a positive correlation between quantitative expression and adhering activity of CR1 (r = 0.79, P <0.01). Compared with those on preoperative day (0.738 ± 0.23, 45.4 ± 4.9), the number and adhering activity of CR1 in patients with gallbladder carcinoma decreased greatly on the third postoperative day (0.310 ± 0.25, 31.8 ± 5.1) (P <0.01), and on the first postoperative week (P> 0.05). The number and adhering activity of CR1 recovered in the second postoperative week (0.740 ± 0.24, 46.8 ± 5.9) (P < P <0.01) and increased greatly in the third postoperative week (0.858 ± 0.35, 52.7 ± 5.8) (P <0.01) in comparison with those on the preoperative day and inthe first postoperative week. The number and adhering activity of CR1 of gallbladder carcinoma patients within filing , adjacent lymphogenous and distant organmetastases were significantly lower than those of patients with gallbladder carcinoma patients without them (P <0.01) .Nodifference was observed between the patients withgallbladder carcinoma and healthy individuals in the spotmutation rate of CR1 density gene (x ~ 2 = 0.521, P> 0.05). The distr ibution of expressionwas 67.8% in high expressiongenomic type, 24.8% in moderate expression genomic type, and 7.4% in low expression genomic type. The number and activity of CR1 high expression genomic type gallbladder carcinomas (0.749 ± 0.22, 42.1 ± 6.2) weresignificantly lower than those of healthy individuals (1.240 ± 0.29, 63.9 ± 7.2), and were also significantly lower than those of healthy individuals (0.921 ± 0.23, 54.8 ± 7.1), but nodifference was observed between the number and adhering activity of CR1 lower expression genomic type gallbladder carcinomas ± 0.18, 44.3 ± 5.5) and those of healthy individuals (0.610 ± 0.20, 45.8 ± 5.7) (P> 0.05) .CONCLUSION: Defective expression of CR1 in gallbladder carcinoma is mostly acquired through central peripheral mechanisms, The changes in CR1 quantitative expression and adhering activity are consanguineously related to the development and metastasis in gallbladder carcinoma.