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使用一定浓度的牛心朴总生物碱苦楝果提取物处理的构树枝条作为天牛成虫的食物 ,比较了其对桑天牛产卵量及卵孵化率的影响。在室温下 ,将成对的天牛放在盖有铁纱网的直径 2 5cm高 2 5cm的塑料桶中进行试验。在 15d试验中 ,用 30 0 μg·mL- 1 的牛心朴总生物碱、10 0 0 μg·mL- 1 的苦楝果提取物处理枝条的两处理组的各 7对天牛其日平均产卵量分别为 3 5 3± 4 18个和 6 73± 3 82个 ,而对照组 7对天牛的日均产卵量为 13 73± 2 90个。经t—检验处理组与对照组之间在日均产卵量方面差异极为显著。 30 0 μg·mL- 1 的牛心朴总生物碱和 10 0 0 μg·mL- 1 的苦楝果提取物对桑天牛卵孵化有显著影响。其影响表现在两方面 :(1)孵化率降低。成虫取食 30 0 μg·mL- 1 牛心朴总生物碱和 10 0 0 μg·mL- 1 苦楝果提取物处理的构树枝条后产的卵最终孵化率分别为 77 36 %、87 12 %。而对照组的最终卵孵化率为 99 5 %。 (2 )其孵化期延长。牛心朴处理组的最终孵化期为 17天、苦楝果处理组的最终孵化期为 15d、而对照组的最终孵化期为 11d。本文还结合我国牛心朴和苦楝的资源情况讨论了利用它们的提取物研究开发防治害虫的行为控制新方法
Using a certain concentration of bovine total alkaloids Melia azedarach extract tree branches treated as adult beetles food, compare their effects on oviposition and hatching rate of Morus alba. Pairs of celastrus were placed in plastic buckets with a diameter of 2.5 cm and a height of 25 cm covered with iron gauze at room temperature. In the 15d test, the average daily fecundity of 7 pairs of beetles of the two treatment groups treated with 30 0 μg · mL -1 bovine total alkaloids and 100 μg · mL -1 neem extract Respectively, which were 353 ± 4 18 and 6 73 ± 3 82, respectively, while the average daily fecundity of 7 pairs of beetles in the control group was 13 73 ± 2 90. There was significant difference in average daily fecundity between t-test group and control group. The total alkaloids of Radix Puerariae of 30 0 μg · mL-1 and the Melia azedarach extract of 100 μg · mL-1 had significant effects on egg hatching of Morus alba. Its impact is manifested in two aspects: (1) reduced hatching rate. The final egg hatching rates of adult tree pests treated with 30 0 μg · mL -1 total berberine alkaloids and 100 μg · mL -1 neem extract were 77 36% and 87 12%, respectively. In the control group, the final egg hatching rate was 99.5%. (2) The incubation period is extended. The final incubation period of Artemisia przewalskii was 17 days, that of Melia azedarach was 15 days, and that of the control group was 11 days. In this paper, we also discussed the new methods of controlling pests by using their extracts according to the resources of our country.