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目的探讨款冬花挥发油对肺纤维化大鼠肺功能及细胞外基质代谢等的影响。方法采用博莱霉素气管注射建立大鼠肺纤维化模型。60只Wistar大鼠分为6组(每组10只):对照组、模型组、款冬花挥发油高、中、低剂量组及地塞米松组,以动物肺功能检测系统检测肺功能,以分光光度计检测肺组织中羟脯氨酸(Hyp)的含量。结果与对照组比较,模型组大鼠肺系数明显升高(P<0.01);吸气气道阻力(RL)、吸气总气道阻力(Re)及用力呼气时间(PET)显著升高(P<0.05或P<0.01);肺顺应性(Cdyn)、第0.3秒用力呼气容积(FEV0.3)、FEV0.3与用力肺活量(FVC)的比值(FEV0.3/FVC)、最大呼气中期流速(MMF)、用力最大呼气流速(PEF)显著降低(P<0.05或P<0.01);肺组织Hpy含量明显升高(P<0.01)。与模型组比较,款冬花挥发油高、中剂量组及地塞米松组的肺系数明显降低(P<0.05或P<0.01);款冬花高剂量组及地塞米松组RL、Re及PET显著降低(P<0.05或P<0.01);Cdyn、FEV0.3、FEV0.3/FVC、MMF、PEF增加(P<0.05或P<0.01);款冬花各剂量组及地塞米松组肺组织Hyp的含量明显降低(P均<0.01)。结论款冬花挥发油能够改善肺纤维化大鼠肺功能及肺组织细胞外基质的代谢。
Objective To investigate the effect of the essential oil of Coltsfoot flower on lung function and extracellular matrix metabolism in rats with pulmonary fibrosis. Methods Pulmonary fibrosis model was established by intratracheal injection of bleomycin. 60 Wistar rats were divided into 6 groups (10 in each group): control group, model group, high, medium and low dose of Coltsfoot flower oil group and dexamethasone group, lung function test system was used to detect lung function, Photometric detection of lung tissue hydroxyproline (Hyp) content. Results Compared with the control group, the lung coefficient of the model group was significantly increased (P <0.01), and the inspiratory airway resistance (RL), inspiratory total airway resistance (Re) and forced expiratory time (PET) (P <0.05 or P <0.01); lung compliance (Cdyn), forced expiratory volume at 0.3 second (FEV0.3), FEV0.3 and forced vital capacity (FVC) (P <0.05 or P <0.01), and the level of Hpy in lung tissue was significantly increased (P <0.01). Compared with the model group, the lung coefficient of high and middle dose group and dexamethasone group were significantly decreased (P <0.05 or P <0.01); RL, Re and PET of high dose group and dexamethasone group were significantly lower (P <0.05 or P <0.01); Cdyn, FEV0.3, FEV0.3 / FVC, MMF and PEF increased (P <0.05 or P <0.01) Content was significantly lower (P all <0.01). Conclusion Volatile Oil of Coltsfoot flower can improve pulmonary function and lung tissue metabolism of extracellular matrix in rats with pulmonary fibrosis.