To elucidate mechanisms regulating ascorbic acid(AsA) biosynthesis and accumulation in the fruit and leaves of black currants,As A and the activities of key enzymes in the ascorbate–glutathione(As A–GSH) cycle were measured from fruit set to fruit ripening during fruit thinning treatments of three common commercial black currant cultivars that differed in their As A levels:’Risager’(low),’Brodtrop’(medium) and ’Adelinia’(high).Treatments were 50% fruit reduction(50% of total fruit set) by hand,control was no thinning.Fruit thinning treatment significantly increased As A content in fruit of all three cultivars from weeks 2 to 8,significantly decreased As A content in leaves from weeks 3 to 8.Dehydroascorbate reductase and monodehydroascorbate reductase activities in fruit and leaves had a similar pattern,increasing during week 2,rose until they peaked in week 4.Ascorbate peroxidase activity in fruit in the thinning treatment was slightly lower than in the control.Fruit thinning was shown to be a good model to test As A biosynthesis regulation and accumulation in black currants.The results from our study provided strong evidence that As A–GSH cycle involved in As A synthesis and accumulation in fruit. To elucidate mechanisms regulating ascorbic acid (AsA) biosynthesis and accumulation in the fruit and leaves of black currants, As A and the activities of key enzymes in the ascorbate-glutathione (As A-GSH) cycle were measured from fruit set to fruit ripening during fruit thinning treatments of three common commercial black currant cultivars that differed in their As A levels: ’Risager’ (low), ’Brodtrop’ (medium) and ’Adelinia’ (high) .Treatments were 50% fruit reduction (50% of total fruit set) by hand, control was no thinning. Fruit thinning treatment significantly increased As A content in fruit of all three cultivars from weeks 2 to 8, significantly decreased As A content in leaves from weeks 3 to 8. Dehydroascorbate reductase and monodehydroascorbate reductase activities in fruit and leaves had a similar pattern, increasing during week 2, rose until they peaked in week 4. Ascorbate peroxidase activity in fruit in the thinning treatment was slightly lower than in the control. Flow thinning was shown to be a good model to test As A biosynthesis regulation and accumulation in black currants. The results from our study provided strong proof that As A-GSH cycle involved in As A synthesis and accumulation in fruit.