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男性不育病因较为复杂。本文应用放射免疫分析法(RIA)测定了生育、不育和无精症男性精浆铜蓝蛋白(CP)的含量。并对精浆中CP含量与精液中脓细胞数量及抗精子抗体(AsAb)之间的关系进行了探讨,现报道如下。 对象和方法 一、对象: (一)生育组:妻子受孕20～30周的健康男性43例,年龄20～26岁,平均年龄24岁。 (二)不育组:婚后3～7年,性生活正常,精液常规检查精子数正常的36例(排除女方不育),年龄25～30岁,平均年龄23岁。 (三)无精症组:31例。精液常规检查无精子。年龄21～32岁,平均年龄27。 二、方法: (一)全部受检者禁欲一周后,手淫法采集全部新鲜精液,37℃完全液化后进行常规检查及脓细胞计数。分离精浆-20℃保存。同时抽静脉血分离血清-20℃保存。 (二)试剂盒:铜蓝蛋白(RIA)试剂盒由上海放射免疫分析技术研究所提供。操作按说明书。抗精子抗体试剂由南京军区总医院免疫室提供。
The cause of male infertility is more complicated. In this paper, radioimmunoassay (RIA) was used to determine the content of ceruloplasmin (CP) in fertile, infertile and azoospermic men. And the content of seminal plasma CP and seminal fluid in the number of pus and antisperm antibody (AsAb) the relationship between the are reported as follows. Subjects and methods First, the object: (a) fertility group: pregnant women 20 to 30 weeks pregnant wife 43 cases, aged 20 to 26 years, mean age 24 years. (B) infertility group: 3 to 7 years after marriage, sexual life is normal, normal sperm routine examination of sperm count of 36 cases (excluding female infertility), aged 25 to 30 years old, mean age 23 years old. (C) Azoospermia group: 31 cases. Semen routine examination without sperm. Age 21 ~ 32 years old, average age 27. Second, the method: (a) all subjects were abstinence after a week, masturbation method to collect all fresh semen, 37 ℃ complete liquefaction after routine examination and pus cell count. Separation of seminal plasma stored at -20 ℃. At the same time pumping venous blood serum-20 ℃ preservation. (B) Kit: Ceruloplasmin (RIA) kit by the Shanghai Institute of radioimmunoassay provides. Operation according to the instructions. Anti-sperm antibody reagent provided by the Nanjing Military Region General Hospital immunization room.