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人外周血液中,T淋巴细胞的酸性α-醋酸萘酚酯酶(Acidα-Naphthyl-Acetate Esterase简称ANAE)活性呈阳性,B淋巴细胞则呈阴性。有人提出可以按ANAE活性的有无、来判定T淋巴细胞。作者对这种报导进行了探讨,并对某些淋巴细胞增生性疾病的病态淋巴细胞的ANAE活性进行了研究。染色方法血液涂片或印片标本乾燥后,用2.5%戊二醛(pH7.2)4℃下固定10分钟后水洗。染色液的配制是,将4%酸性副品红以2N HCl溶液溶解后取1分与4%亚硝酸钠1分混合。再取此混合液5ml
In human peripheral blood, the activity of Acid alpha-Naphthyl-Acetate Esterase (ANAE) is positive for T lymphocytes and negative for B lymphocytes. It has been proposed that T lymphocytes can be determined based on the presence or absence of ANAE activity. The authors discussed this report and studied the ANAE activity of diseased lymphocytes in certain lymphoproliferative disorders. Methods of Staining After the blood smears or printed specimens are dried, they are washed with 2.5% glutaraldehyde (pH 7.2) for 10 minutes at 4°C and then washed. The staining solution was prepared by dissolving 4% acid parafuchsin in 2N HCl solution and then mixing 1 point with 1% sodium nitrite. Then take this mixture 5ml