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目的 :建立感染幽门螺杆菌 (Helicobacterpylori,H pylori)SS1株BALB/c小鼠感染模型 ,研究H pylori胃内定植及胃黏膜病理变化。 方法 :BALB/c小鼠胃内分别接种体外培养的H pyloriSS1株 (实验组 )或PBS(对照组 ) ,组织学方法评价H pylori定植及胃黏膜病理变化。结果 :所有对照组小鼠胃组织未见H pylori定植 ,胃组织也未见明显的炎症反应 ;而所有实验组小鼠在感染H pylori 12周后 ,胃黏膜表面的黏液层及胃小凹顶端可见大量H pylori,胃体及胃窦交界处、胃体及胃底交界处最多 ;胃组织可见到不同程度的炎性反应 ,感染H pylori 2 4周后 ,胃组织炎性反应加重。结论 :用胃内接种方法建立了小鼠H pylori感染及其相关性胃炎的模型。
Objective: To establish a BALB / c mouse model of infection with Helicobacter pylori (SS) strain SS1 and investigate the pathological changes of gastric mucosa and H pylori colonization. Methods: H pylori SS1 (experimental group) or PBS (control group) were inoculated into the stomach of BALB / c mice in vitro and histopathologically evaluated for H pylori colonization and gastric mucosal pathological changes. RESULTS: No Hpylori colonization was found in gastric tissues of all the control mice, and no obvious inflammatory reaction was observed in gastric tissues. However, in all experimental mice infected with H pylori for 12 weeks, the mucous layer on the surface of gastric mucosa and the top of gastric pits A large amount of H pylori was observed. The junction of gastric body and gastric antrum was the largest at the junction of gastric body and gastric fundus. Different degrees of inflammatory reaction were observed in gastric tissue. After 4 weeks of H pylori infection, the inflammatory reaction of gastric tissue was aggravated. Conclusion: The model of H pylori infection and related gastritis in mice was established by intragastric inoculation.