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目的探讨螺旋藻精片安全性毒性实验。方法采用小鼠急性经口毒性试验、Ames试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验及大鼠30天喂养试验。结果螺旋藻对小鼠急性经口毒性实验结果大于15000mg/kgBW,体内体外致突变实验结果均为阴性,30天喂养试验,以3334、5000、6667mg/kgBW(分别相当成人日用量的50、75、100倍)3个剂量组的样品掺入饲料中,连续喂养30天,试验期间动物生长发育良好,各剂量组的动物体重、增重量和进食量及食物利用率组间相比无差异,与对照组比较,差异均无显著性(P>0.05);各剂量组血常规和生化检测指标、脏器重量及脏器/体重比值与对照组比较,差异均无显著性(P>0.05);病理组织学检查结果,各实验组大鼠肝、肾、胃肠、脾、睾丸和卵巢等组织均未见有病理改变。结论本试验提示螺旋藻对大鼠30天毒性实验及小鼠毒性试验未产生不良影响。
Objective To investigate the safety of spirulina tablets. Methods Acute oral toxicity test, Ames test, mouse bone marrow polychromatic erythrocyte micronucleus test and mouse sperm abnormality test and 30-day feeding test in rats were performed. Results Spirulina acute oral toxicity test results in mice greater than 15000mg / kgBW, in vivo mutagenic results in vitro were negative, 30 days feeding test to 3334,5000,6667mg / kgBW (respectively, the amount of adult daily 50,75 , 100 times). The samples of three dose groups were fed into the feed continuously for 30 days. The animals grew well during the experiment. There was no difference in body weight, weight gain, food intake and food utilization among the three groups. Compared with the control group, the differences were not significant (P> 0.05). There was no significant difference in blood and biochemical indexes, organ weight and organ / body weight ratio between the two groups (P> 0.05) The histopathological examination showed no pathological changes in the liver, kidney, gastrointestinal tract, spleen, testis and ovary in all experimental groups. Conclusion This test indicates that Spirulina had no adverse effect on the 30-day toxicity test and the mouse toxicity test.