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[目的]探索浙江省畲汉两族原发性高血压患者血清血管紧张素转换酶(ACE)水平与ACE基因多态性的关系。[方法]采用聚合酶链反应(PCR)检测215例畲汉两族原发性高血压(病例组)及正常人群血中ACE基因16内含子I/D多态标记;采用紫外分光光度法测定血清ACE水平;运用多元线性回归分析血清ACE水平与ACE基因型的关系。[结果]浙江畲汉两族血清ACE水平均为病例组高于对照组(P﹤0.001)。在两族的病例组和对照组中,不同ACE基因型之间血清ACE水平的差别有统计学意义(P﹤0.001),两两比较,各组均为DD型﹥ID型﹥II型。多元线性回归显示DD基因型和ID基因型相对于II基因型能使血清ACE水平增加(P﹤0.001)。[结论]原发性高血压患者血清ACE水平较对照组高;畲汉两族的民族因素与血清ACE水平无关;两族中DD及ID基因型的个体血清ACE水平较II基因型高。
[Objective] To explore the relationship between the serum level of angiotensin-converting enzyme (ACE) and ACE gene polymorphism in essential hypertension patients in Shehan and Han nationality of Zhejiang Province. [Method] Polymerase chain reaction (PCR) was used to detect the polymorphism of ACE gene 16 intron I / D polymorphism in blood samples from 215 cases of SHE and essential hypertension in normal people. The ultraviolet spectrophotometry Serum ACE levels were measured. The relationship between serum ACE levels and ACE genotypes was analyzed by multivariate linear regression analysis. [Results] Serum ACE levels in both Shehan and Han Chinese were higher in case group than in control group (P <0.001). There were significant differences in serum ACE levels between different ACE genotypes in the two ethnic groups and in the control group (P <0.001). For any pairwise comparisons, each group was DD type> ID type> II type. Multiple linear regression showed that DD genotype and ID genotype increased serum ACE levels relative to genotype II (P <0.001). [Conclusion] The serum ACE level of patients with essential hypertension is higher than that of the control group. The ethnic factors of She-Han two ethnic groups have nothing to do with the level of serum ACE. The serum ACE levels of DD and ID genotypes of two ethnic groups are higher than those of II genotypes.