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为了探讨近红外光谱分析技术作为快速分析技术应用于检测万古霉素血药浓度的可行性,配制浓度梯度在2~98μg/m L的万古霉素绵羊血浆样品,采用透射模式采集样品在10 000~4 000 cm-1的透射光谱。利用K-S方法将样品集划分为校正集和验证集,通过预处理方法的优化选择最优的光谱预处理方法,建立万古霉素血药浓度分析模型并对模型进行评价。利用一阶导数Norris平滑对光谱进行预处理后建立的PLS模型,模型的RMSEP=3.182μg/m L,Rp=0.993 9,RPD=8.6,可用于血浆中万古霉素的定量分析。近红外光谱分析技术用于血浆中万古霉素浓度具有很高的准确性,可用于体外血浆样品中万古霉素浓度的检测。
In order to investigate the feasibility of using near-infrared spectroscopy as a rapid analytical technique in the determination of vancomycin plasma concentrations, vancomycin sheep plasma samples with concentration gradients between 2 and 98 μg / m L were prepared, and their transmission spectra were collected at 10 000 ~ 4 000 cm-1 transmission spectrum. The K-S method was used to divide the sample set into a calibration set and a verification set. The optimum spectral pretreatment method was optimized by pretreatment method to establish a model of vancomycin plasma concentration analysis and evaluate the model. The PLS model was established by first order Norris smoothing spectrum pretreatment. The model has RMSEP = 3.182μg / m L, Rp = 0.993 9, RPD = 8.6 and can be used for the quantitative analysis of vancomycin in plasma. The near-infrared spectroscopy technique has high accuracy for the determination of vancomycin in plasma and can be used for the detection of vancomycin in plasma samples.