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目的使用小鼠验证这样一个假设:外界病毒浸入诱发心肌炎时,机体的干细胞将进入心脏提高心肌的抗病毒能力。方法雄性BALB/c小鼠分为三组:小鼠胚胎干细胞对照组(ES),心肌炎病毒组(EM CV)及EM-CV加ES治疗组。通过尾静脉注射,令小鼠立即感染病毒。小鼠死亡率,炎性细胞浸润及心肌坏死等为观察指征。干细胞的游走及分化等通过免疫荧光法来验证。结果给予干细胞后的小鼠的存活率明显高于生理盐水对照组,炎性细胞侵润及心肌坏死亦明显低于生理盐水对照组。免疫荧光法表明,干细胞进入心肌并分化成新的心肌细胞。结论干细胞能明显提高心肌炎小鼠的存活率,减少心肌组织的坏死。同时,亦证明当心脏遭受病毒的侵入后,干细胞通过某种机理修复或再生心肌细胞,从而提高组织的抗病毒能力。
Purpose To confirm the hypothesis by using mice that the body’s stem cells will enter the heart and increase the antiviral ability of the myocardium when the virus enters the myocardium. Methods Male BALB / c mice were divided into three groups: mouse embryonic stem cell control group (ES), myocarditis virus group (EM CV) and EM-CV plus ES treatment group. Through the tail vein injection, the mouse immediately infected with the virus. Mouse mortality, inflammatory cell infiltration and myocardial necrosis were observed indications. Stroke and differentiation of stem cells were verified by immunofluorescence. Results The survival rate of the mice given the stem cells was significantly higher than that of the saline control group. Inflammatory cell infiltration and myocardial necrosis were also significantly lower than those of the saline control group. Immunofluorescence showed that stem cells enter the myocardium and differentiate into new cardiomyocytes. Conclusion Stem cells can significantly improve the survival rate of myocarditis mice and reduce the necrosis of myocardial tissue. At the same time, it is also proved that when the heart is invaded by the virus, the stem cells repair or regenerate the cardiomyocytes through some mechanism to improve the anti-virus ability of the tissue.