论文部分内容阅读
目的了解天津地区急性下呼吸道感染儿童中人博卡病毒(HBoV)的感染情况及病毒基因特性。方法采集2008年9月-2009年5月急性下呼吸道感染住院儿童的鼻咽吸取物标本202份,进行PCR扩增来检测HBoVNP-1基因,对VP1及VP2基因进行扩增及序列测定,与GenBank中登录的序列进行比较,分析序列特征并绘制种系发生树,同时采用多重PCR方法检测其他12种呼吸道病毒及WU多瘤病毒。结果 202份标本中,6.9%(14/202)为HBoV阳性,所有阳性患儿的年龄均≤6岁,6月～12月龄患儿感染率最高,为14.3%(6/42);所有感染者均为肺炎和喘息性支气管炎患儿,各月份均有检出;HBoV与其他病毒的混合感染率为85.7%(12/14),其中58.3%(7/12)为3种以上呼吸道病毒混合感染;VP1/VP2基因种系发生树分析表明13株HBoV均属于Ib簇,同源性分析表明,天津地区HBoV VP1基因与从GenBank下载的I型序列比较,核酸及氨基酸的同源性分别为99.0%～99.8%及99.0%～99.9%。结论天津地区存在HBoV流行,感染者多为<6岁儿童,HBoV与其他呼吸道病毒混合感染常见,HBoV病毒均属于Ib簇。
Objective To investigate the prevalence of human Boca and Bovine (HBoV) infection in children with acute lower respiratory tract infection in Tianjin and the genetic characteristics of the virus. Methods Totally 202 samples of nasopharyngeal aspirates from hospitalized children with acute lower respiratory tract infection from September 2008 to May 2009 were collected for PCR amplification to detect HBoVNP-1 gene. The VP1 and VP2 genes were amplified and sequenced. The sequences registered in GenBank were compared, the sequence characteristics were analyzed and the phylogenetic tree was drawn, and other 12 kinds of respiratory viruses and WU polyoma virus were detected by multiplex PCR. Results Of the 202 specimens, 6.9% (14/202) were positive for HBoV, all of them were ≤6 years old, and the highest infection rate was 14.3% (6/42) in all patients Infected persons were all children with pneumonia and asthmatic bronchitis, and were detected in each month. The mixed infection rate of HBoV with other viruses was 85.7% (12/14), of which 58.3% (7/12) were more than 3 kinds of respiratory tract Virus mixed infection. The phylogenetic tree analysis of VP1 / VP2 showed that 13 HBoV belonged to Ib cluster. Homology analysis showed that the homology of nucleic acid and amino acid between HBoV VP1 gene in Tianjin and type I sequence downloaded from GenBank Respectively 99.0% -99.8% and 99.0% -99.9%. Conclusions The prevalence of HBoV in Tianjin is high, most of whom are children <6 years of age. HBoV is commonly found in mixed infections with other respiratory viruses, and HBoV belongs to group Ib.