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应用SRAPI、SSR标记构建红麻分子遗传连锁图,以半野生种Ga42(源自加纳)和栽培种阿联红麻(源自埃及)杂交产生的203株F2代分离群体作为作图群体。筛选出多态性好的27对SRAP引物组合和5个ISSR引物,对F2作图群体进行PCR扩增,共产生108个多态性条带,平均每个引物组合产生3.4个多态性条带,最多的可产生8条多态性条带。应用MAPMAKER/EXP3.0软件对108个多态性条带进行遗传连锁分析,被分为16个连锁群(L0D≥3.0),初步构建了首张红麻遗传连锁图谱,该图谱总长为1336.6 cM,平均两个标记位点间距为17.82cM。本文还讨论了图谱构建存在的偏分离有关问题与对策,可为进一步构建较高密度、分布均匀的遗传图谱及基因定位奠定了良好的基础。
The genetic linkage map of Kenaf was established by SRAPI and SSR markers. 203 F2 generation segregation populations derived from hybridization of Ga42 from Ghana and Kenaf from cultivars from Egypt were used as mapping population. 27 pairs of SRAP primer combinations and 5 ISSR primer pairs with good polymorphism were screened and F2 population was amplified by PCR to generate 108 polymorphic bands with an average of 3.4 polymorphic bands per primer combination With up to 8 polymorphic bands can be generated. Genetic linkage analysis of 108 polymorphic bands was performed using MAPMAKER / EXP3.0 software and was divided into 16 linkage groups (L0D≥3.0). The first kenaf genetic linkage map was constructed and the total length of this map was 1336.6 cM , The average spacing between two markers was 17.82cM. This paper also discussed the problems and countermeasures of partial segregation in the construction of the map, which laid a good foundation for further constructing the genetic map and gene mapping with higher density and uniform distribution.