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以玉米自交系A188为试材,将含有Bar基因和Bt基因的质粒pBW3导入玉米幼胚内,获得了转基因抗性苗。抗性植株点杂交及Southern杂交结果显示,该基因已整合到抗性玉米植株体内,外源基因在玉米基因组内有4个拷贝。
A maize inbred line A188 was used as the test material, and the plasmid pBW3 containing Bar gene and Bt gene was introduced into immature embryos of maize to obtain transgenic resistant seedlings. The results of dot blot hybridization and Southern blotting showed that this gene was integrated into resistant maize plants and the exogenous genes contained 4 copies in the maize genome.