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为研究外源豌豆铁蛋白基因(Pea-Fer)的导入和表达对水稻植株和子粒重要矿质元素的积累所产生的影响,本试验以原始受体亲本粳稻品种秀水11为轮回亲本,外源豌豆铁蛋白转基因纯系Fer34为非轮回亲本,采取连续回交,结合GUS标记基因辅助选择技术,在BC6F3获得含Pea-Fer的Fer34-XS11,它与秀水11构成一对近等基因系。并利用该近等基因系,研究Pea-Fer基因导入对水稻植株不同生长发育阶段(幼苗期、分蘖期、成熟期)的根、茎(叶鞘)、叶及子粒不同部分(谷壳、米糠、糙米、精米)的主要矿质元素(Fe、Ca、Mn、Zn)积累的影响。结果表明:外源豌豆铁蛋白基因(Pea-Fer)的导入,使得水稻植株在不同生长发育时期的根、茎(叶鞘)、叶等器官中的Fe含量较对照品种秀水11明显增加,而对于Ca、Mn和Zn的含量并没有显著影响;同时,水稻子粒中Fe含量积累也有较高提升,而Ca、Mn和Zn的积累却未出现显著变化。这为深入开展转基因富铁水稻新种质的研究和利用提供了一定依据。
In order to study the effect of introduction and expression of exogenous pea-fer gene on the accumulation of important mineral elements in rice plants and grain, we used the original recipient parent Japonica rice Xiushui 11 as the recurrent parent, exogenous pea The Fer34 ferritin Fer34-XS11 with Pea-Fer was obtained from BC6F3 by continuous backcrossing with continuous backcrossing, which formed a pair of near-isogenic lines with Xiushui 11. Using the near-isogenic lines, we studied the effect of Pea-Fer gene transference on different parts of the root, stem (leaf sheath), leaf and grain at different growth and development stages (seedling stage, tillering stage and mature stage) of rice plants (chaff, rice bran, Brown rice, polished rice) the main mineral elements (Fe, Ca, Mn, Zn) accumulation. The results showed that the introduction of exogenous pea-Fer gene resulted in a significant increase of Fe content in roots, stems (leaf sheaths) and leaves of rice plants compared with that of Xiushui 11 at different growth stages, Ca, Mn and Zn contents did not change significantly. Meanwhile, Fe accumulation in rice grains also increased, while Ca, Mn and Zn accumulation did not change significantly. This provides a certain basis for further research and utilization of new germplasm of transgenic iron-rich rice.