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对籼型杂交水稻B优827苗期进行不同PEG浓度的水分胁迫处理,研究不同水分胁迫下超氧化物歧化酶(SOD)、过氧化物酶(POD)同功酶的变化.通过对不同浓度处理4d后的叶片进行同功酶电泳,发现SOD,POD同功酶在7.5%PEG下开始出现新的条带,并且POD同功酶在15%PEG,20%PEG下分别有新条带的增减.对7.5%PEG和20%PEG处理下0～4d的叶片进行同功酶电泳比较,发现7.5%PEG处理下SOD、POD第2天开始出现新条带.20%PEG处理下SOD到第4天出现新条带,POD在胁迫的第1天就出现了新同功酶条带,并随着胁迫时间的增加其同功酶条带有增有减.
Under different water stress, the changes of superoxide dismutase (SOD) and peroxidase (POD) isoenzymes under different water stress treatments were studied under different PEG concentrations at seedling stage of indica hybrid rice B You 827. The effects of different concentrations After 4 days of treatment, isozyme electrophoresis showed that SOD and POD isozymes began to appear new bands under 7.5% PEG, and POD isozymes had new bands at 15% PEG and 20% PEG, respectively The results showed that 7.5% PEG treatment and SOD enzyme activity in 0 ~ 4d under 0% ~ 4% A new band appeared on the 4th day. POD showed a new band of the same enzyme on the first day of stress, and it increased or decreased with the increase of the stress time.