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由于Kidd血型抗原明显的致免疫性及其系统抗体的不稳定性,所以,该抗原在输血实践中很重要。由于每个细胞上抗原位点数相当少,致Kidd抗原的纯化受到阻碍。作者介绍的改良DOT-BLOT纯化法,能够克服上述问题,并可识别人类红细胞的Kidd抗原。方法是:把经菠萝酶处理、以抗-Jk~a,抗-JK~b或抗-JK~3致敏的Panocell-10人类红细胞铺设在已固有抗-人IgG的ZP-膜上,置37℃孵育30分钟,用生理盐水和含7.5g/L辛基-β-D-吡喃葡糖苷的生理盐水洗涤、浸没,以除去未粘附的红细胞和抗原-抗体复合物以外的其他红细胞成分。留在Z-P-膜上,结合了抗体的特异血型抗原,用10g/L+二烷基硫酸钠(SDS)洗脱,洗脱液经十二烷基硫酸
Due to the obvious immunogenicity of Kidd blood group antigen and the instability of its systemic antibodies, this antigen is important in blood transfusion practice. Purification of Kidd antigen is hampered by the relatively small number of antigenic sites per cell. The improved DOT-BLOT purification method introduced by the authors overcomes these problems and identifies the Kidd antigen of human erythrocytes. The method is: Panocell-10 human erythrocytes treated with pineapple enzyme and sensitized with anti-Jk-a, anti-JK-b or anti-JK-3 are plated on the ZP-membrane which is already provided with anti-human IgG Incubate at 37 ° C for 30 minutes, wash with physiological saline and physiological saline containing 7.5 g / L octyl-β-D-glucopyranoside and immerse to remove non-adherent erythrocytes and other red blood cells other than the antigen-antibody complex ingredient. Left in the Z-P-membrane, combined with the specific antibody of the blood group antigen, with 10g / L sodium dialkyl sulfate (SDS) elution, the eluent by dodecyl sulfate