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Our study aimed to investigate the protective effects of Holothurian intestines(HI) on NSAIDs-induced gastric mucosal damage and the possible mechanism. At first, 60 male Wistar rats were induced of gastric lesions with indomethacin(IDM, 30 mg kg~(-1)). The rats were pretreated for 15 consecutive days with saline, sucralfate, or HI(0.4g kg~(-1) d-1, 0.8 g kg~(-1) d~(-1) and 1.6 g kg~(-1) d~(-1)) prior to IDM treatment, followed by evaluations of macroscopic damage and microscopic features; and investigation of the levels of inflammatory cytokines, oxidative stress parameters, gastric mucosal prostaglandin E2(PGE2) and total hexosamine in tissues. The expression of COX-1 and COX-2 m RNA in the gastric tissue were determined by quantitative polymerase chain reaction(q PCR). Pathological gastric ulcer indexes, levels of pro-inflammatory cytokines(IL-1β, IL-17, TNF-α) and lipid peroxidation were significantly decreased in HI-treated groups, whereas the levels of protective factors(TGF-β, GSH, SOD activity and PGE2) were significantly elevated especially in the group with HI 1.61 g kg~(-1) d~(-1)(P < 0.05). Furthermore, the expression of COX-2 mRNA decreased significantly in HI groups(P < 0.05). The study investigates that holothurian intestines may act as a kind of marine medicine which have protective effect on IDM-induced gastric ulcer, which could be a dietary preventive agent for the prevention of gastric damage.
Our study aimed to investigate the protective effects of Holothurian intestines (HI) on NSAIDs-induced gastric mucosal damage and the possible mechanism. At first, 60 male Wistar rats were induced of gastric lesions with indomethacin (IDM, 30 mg kg -1 ). The rats were pretreated for 15 consecutive days with saline, sucralfate, or HI (0.4g kg -1 d -1, 0.8 g kg -1 d -1 and 1.6 g kg ~ (-1) d -1) prior to IDM treatment, followed by evaluations of macroscopic damage and microscopic features; and investigation of the levels of inflammatory cytokines, oxidative stress parameters, gastric mucosal prostaglandin E2 (PGE2) and total hexosamine in tissues. The expression of COX-1 and COX-2 m RNA in the gastric tissue were determined by quantitative polymerase chain reaction (q PCR). Pathological gastric ulcer indexes, levels of pro- inflammatory cytokines (IL-1β, IL- TNF-α) and lipid peroxidation were significantly less in HI-treated groups, while the levels of protective factors (TGF-β , GSH, SOD activity and PGE2) were significantly elevated in the group with HI 1.61 g kg -1 d -1 (P <0.05). Furthermore, the expression of COX-2 mRNA decreased significantly in HI The study investigates that holothurian intestines may act as a kind of marine medicine which have protective effect on IDM-induced gastric ulcer, which could be dietary preventive agent for the prevention of gastric damage.