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目的建立类鼻疽杆菌(Burkholderia pseudomallei)慢性感染BALB/c小鼠模型并评价其稳定性。方法采用0.01 m L洗涤后的高、中、低剂量的类鼻疽杆菌液(菌量分别为3.3×104、3.3×103、3.3×102CFU)滴鼻感染BALB/c小鼠,密切监测42 d,记录小鼠的生存率,探索合适的攻毒剂量。在低剂量细菌(3.3×102CFU)感染小鼠后21、28、35、42 d,计数小鼠血液、肝脏、脾脏和肺脏的细菌定植量,ELISA检测血清炎性因子TNF-α、IFN-γ的表达和血清类鼻疽杆菌特异性Ig G抗体滴度,观察肝脏、脾脏和肺脏等主要器官的病理学改变,评价动物模型的稳定性。结果低剂量类鼻疽杆菌滴鼻感染BALB/c小鼠后,小鼠42 d生存率为60%。期间小鼠体质量呈下降趋势,血液、肝脏、脾脏和肺脏均有细菌定植,并出现大量炎性细胞浸润、多灶性坏死和脓肿等病理改变;类鼻疽杆菌血清中特异性Ig G抗体滴度从感染后21 d起持续增高;实验组小鼠血清炎性因子TNF-α和IFN-γ表达明显高于对照组(P<0.05)。结论成功建立了稳定的类鼻疽杆菌慢性感染BALB/c小鼠模型。
Objective To establish a BALB / c mouse model of chronic infection with Burkholderia pseudomallei and evaluate its stability. Methods BALB / c mice were intranasally infected with high, medium and low doses of B. anthracis solution (volume of 3.3 × 104, 3.3 × 103, 3.3 × 102CFU respectively) The survival rate of mice to explore the appropriate dose of challenge. Bacterial colonies were counted in mice blood, liver, spleen and lungs 21, 28, 35 and 42 days after infection with low doses of bacteria (3.3 × 102CFU). Serum inflammatory cytokines TNF-α, IFN-γ And the serum IgM specific titer of B. anthracis. The pathological changes of major organs such as liver, spleen and lung were observed to evaluate the stability of animal models. Results The low dose of B. anthracis infection in BALB / c mice intranasally infected mice at 42 days survival rate was 60%. During the period, the body weight of mice showed a decreasing trend. Bacterial colonies were found in the blood, liver, spleen and lungs. A large number of pathological changes such as inflammatory cell infiltration, multifocal necrosis and abscess were observed. The titer of specific IgG antibodies against B. anthracis sera The levels of serum inflammatory cytokines TNF-α and IFN-γ in the experimental group were significantly higher than those in the control group (P <0.05). Conclusion A stable BALB / c mouse model of B. anthracis infection was established successfully.