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目的为了解东北地区蜱中携带立克次体的情况。方法应用人工小时布旗法采集东北部分地区不同生境的游离蜱,采用PCR作斑点热立克次体、查菲埃立克体、人粒细胞无形体DNA检测。结果从东北地区全沟硬蜱和森林革蜱检出查菲埃立克体DNA,全沟硬蜱平均阳性率为3.29%,森林革蜱为1.88%;从全沟硬蜱检出人粒细胞无形体DNA,平均阳性率为2.98%;从嗜群血蜱检出斑点热立克次体DNA,其平均阳性率(6.67%)明显高于全沟硬蜱(1.69%)和森林革蜱(2.36%)。结论东北地区全沟硬蜱和森林革蜱可能是查菲埃立克体、人粒细胞无形体的主要携带媒介,嗜群血蜱主要携带斑点热立克次体,提示东北地区存在三种蜱媒立克次体病的自然疫源地。
Objective To understand the situation of tick-borne rickettsia in northeast China. Methods Free ticks collected in different habitats in northeastern China were collected by artificial hour cloth flagging method. The PCR products were used for spotted fever rickettsia, chard ehrlichia and human granulocyte apomixis DNA. Results The results showed that the average positive rate of Iridiform Ephemeralis was 3.29% and that of Forest ephemeris was 1.88%. The positive rate of Myrmecophoromyces foetida The average positive rate of invisible DNA was 2.98%. The average positive rate of spotted hot rickettsia DNA (6.67%) was significantly higher than that of hard tick (1.69% 2.36%). Conclusion Ixodes tricuspidata and forest ticks may be the main carriers of Chafie Ehrlichia and Ammopiptanthus mongholicus in the northeast of China. Media Rickettsia disease natural foci.