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目的 探讨细胞周期蛋白E(cyclinE)、细胞周期蛋白依赖激酶抑制剂 (p2 7KIP1)对成人初治急性白血病 (AL)发展及预后的作用。方法 用流式细胞术检测 6 0例AL及 17例对照cyclinE、p2 7KIP1、多药耐药基因蛋白p170表达及细胞周期分布 ;逆转录 聚合酶链反应检测 4 6例成人AL患者及 17例对照的骨髓cyclinE、p2 7KIP1、多药耐药蛋白的mRNA水平。结果 6 0例AL患者全周期及G2 /M期cyclinE表达均高于对照组 (P <0 0 1或 0 0 5 ) ;p2 7KIP1全周期表达亦均高于对照组 ,但G2 /M期差异无显著性 (P >0 0 5 ) ;cyclinE与p2 7KIP1的表达呈正相关 ;AL患者cyclinE高表达组缓解率(44 8% )低于正常表达组 (77 4 % ) (P <0 0 1) ;复发率 (92 3% )高于正常表达组 (41 7% ) (P =0 0 0 3) ;p2 7KIP1表达对AL缓解率、复发率的影响无统计学意义 (P >0 5 0 ,P =0 89)。结论 cyclinE在成人AL有异常高表达 ,并可能促使AL的发展 ,是AL缓解率、复发率的重要危险因素。
Objective To investigate the effects of cyclin E and p2 7KIP1 on the development and prognosis of acute lymphoblastic leukemia (AL) in adults. Methods Flow cytometry was used to detect the expression of cyclinE, p27KIP1 and multidrug resistance protein p170 in 60 AL patients and 17 controls, and the cell cycle distribution was analyzed by reverse transcription polymerase chain reaction (PCR) in 46 AL patients and 17 controls Of bone marrow cyclinE, p2 7KIP1, multidrug resistance protein mRNA levels. Results The expression of cyclinE in the whole cycle and G2 / M phase in 60 patients with AL was higher than that in the control group (P <0.01 or 0 05), and the expression of p2 7KIP1 in the whole cycle was also higher than that in the control group The expression of cyclinE was positively correlated with the expression of p27KIP1 in AL patients (44.8%, P <0.01) ; The recurrence rate (92.3%) was higher than that in the normal expression group (41.7%) (P = 0 0 0 3); The effect of p2 7KIP1 expression on AL remission rate and recurrence rate was not statistically significant (P> 0.05) P = 0 89). Conclusion CyclinE is highly expressed in adult AL and may promote the development of AL. It is an important risk factor of AL remission rate and recurrence rate.