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目的:分析深圳市2007年食物中毒分离副溶血弧菌血清型,携带毒力基因情况,菌株之间的遗传相关性。方法:对11株副溶血弧菌进行血清学分型,用荧光PCR检测毒力基因tdh,细菌基因组DNA经NotI酶切后进行脉冲场凝胶电泳分析。结果:共有4种血清型,8株菌为血清型O3:K6,所有菌株毒力基因tdh荧光PCR阳性,除两株菌PFGE型相同外,其余菌株有不同的PFGE型。结论:O3:K6血清型菌株是引起副溶血弧菌食物中毒的主要菌型,大部分副溶血弧菌来源分散,实验室分析有助于副溶血弧菌所致食物中毒的预防和控制。
OBJECTIVE: To analyze the serotypes of virulent strains of Vibrio parahaemolyticus isolated from food poisoning in Shenzhen, the virulence genes carrying them and the genetic correlation among strains. Methods: Serotyped 11 strains of Vibrio parahaemolyticus. The virulence gene tdh was detected by fluorescence PCR. The bacterial genomic DNA was digested with NotI and analyzed by pulsed-field gel electrophoresis. Results: There were 4 serotypes, 8 strains were serotype O3: K6, and all strains were positive for tdh by PCR. Except for the same strain of PFGE, the other strains had different PFGE types. Conclusion: O3: K6 serotype strains are the main bacteria causing Vibrio parahaemolyticus food poisoning. Most of the sources of Vibrio parahaemolyticus are scattered. Laboratory analysis is helpful to the prevention and control of food poisoning induced by Vibrio parahaemolyticus.