目的 :建立应用流式细胞术测定红细胞体内存活率的方法。方法 :献血者Rh表型ccDEE ,受血者为CCDEE ,以抗 c为一抗结合ccDEE的红细胞 ,两群细胞按设定的比例混合 ,以山羊抗人标记荧光素的IgGab段为二抗连接抗 c ,经流式细胞仪测定ccDEE表现型红细胞在两群细胞中所占的比例 ,并与实际的比例进行相关性比较。结果 :已知两群细胞数的比例与流式细胞仪测定的比例的相关系数r =99.87。结论 :流式细胞术技术可以体外测定红细胞体内的存活率 Objective: To establish a method of determining the survival rate of erythrocytes by flow cytometry. Methods: The blood donors Rh phenotype ccDEE, the recipients were CCDEE, anti-c as primary antibody combined with ccDEE red blood cells, two groups of cells according to the set ratio of mixing goat anti-human labeled IgGab segment of the second antibody was connected Anti-c, by flow cytometry ccDEE phenotypic red blood cells in the two groups of cells in the proportion, and the actual proportion of the correlation between. Results: The correlation coefficient between the proportion of two groups of cells and the ratio determined by flow cytometry was 99.87. Conclusion: Flow cytometry can be used to determine the survival rate of erythrocytes in vitro