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目的:利用蛋白质二维液相色谱方法建立维吾尔族妇女宫颈癌患者血清蛋白质组特异的蛋白质差异表达谱。方法:收集与制备维吾尔族妇女宫颈癌患者血清样品,采用蛋白质二维液相色谱系统(ProteomeLabTMPF-2D)对血清样品进行分离与数据比较分析。结果:成功建立了基于蛋白质等电点和疏水性特征的血清蛋白质组差异表达图谱,能够鉴别出56个差异表达蛋白位点,其中12个位点的峰值存在显著差异。结论:蛋白质二维液相色谱是建立血清蛋白质组差异表达谱的有效和可行方法,为维吾尔族妇女宫颈癌患者血清蛋白质标志物的筛选开辟了新的途径。
OBJECTIVE: To establish a protein differential expression profile of serum proteome in Uigur women with cervical cancer using protein two-dimensional liquid chromatography. Methods: Serum samples from Uigur women with cervical cancer were collected and analyzed. Proteins were separated and compared with ProteomeLabTMPF-2D. Results: The differential expression profiles of serum proteome based on isoelectric point and hydrophobicity of proteins were successfully established. Fifty-six differentially expressed protein spots were identified, of which twelve peaks were significantly different. Conclusion: Protein two-dimensional liquid chromatography is an effective and feasible method for establishing differential proteomic profiling of serum proteome, which opens a new way for the screening of serum protein markers in Uygur women with cervical cancer.