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鉴于国内还缺乏正常人外周血T细胞亚群的有关资料,我们利用单克隆抗体T_(11)、T_4和T_8及免疫细胞化学技术对一组成年人外周血中T细胞及其亚群分布进行了研究。我国正常人外周血中T细胞、T_4~+细胞及T_8~+细胞在单个核细胞中的比例分别为65.89±8.31;43.38±7.48;25.03±6.41％。无明显性别差异,但50～69岁老年人前期T细胞及T_4~+细胞有所降低,而T_8~+细胞有增高趋势。同时我们还发现血中的T细胞及其亚群存在日间节律性变动,建议应在每天上午8～9时采血。
In view of the lack of normal peripheral blood T lymphocyte subsets in China, we used the monoclonal antibodies T_ (11), T_4 and T_8 and immunocytochemistry to detect the distribution of T cells and their subpopulations in peripheral blood of a group of adults Study. The percentage of T cells, T 4 + cells and T 8 + cells in mononuclear cells of normal people in our country were 65.89 ± 8.31, 43.38 ± 7.48 and 25.03 ± 6.41% respectively. There was no significant gender difference, but the number of T cells and T 4 ~ + cells in the 50 ~ 69 year-old old people decreased, while the T 8 ~ + cells increased. At the same time we also found that blood T cells and their subgroups have rhythmic changes during the day, it is recommended that every day 8 to 9 am blood.