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以电活性钌化合物[Ru(NH3)6]3+为信号传感源,借助碳纳米管构建了高灵敏检测腺苷免标记电化学传感电极(BSA/Apt/CNTs/GC).BSA/Apt/CNTs/GC电极在最佳实验条件下检测腺苷线性范围为5.0×10-11~1.0×10-7mol·L-1,检测下限为2.7×10-11mol·L-1.该传感电极有较高的灵敏度、良好的选择性、重现性和稳定性.与传统标记型适体传感电极相比,其制作简便,也许还适用于其他小分子和蛋白质的检测,有一定的普适性.
A highly sensitive detection of adenosine-free labeled electrochemical sensing electrode (BSA / Apt / CNTs / GC) was constructed with carbon nanotubes using an active ruthenium compound [Ru (NH3) 6] The linear range of adenosine Apt / CNTs / GC was 5.0 × 10-11 ~ 1.0 × 10-7 mol·L-1 and the detection limit was 2.7 × 10-11 mol·L-1 under the best experimental conditions. The electrode has high sensitivity, good selectivity, reproducibility and stability.Compared with the traditional labeled aptamer sensing electrode, its fabrication is simple and may be suitable for the detection of other small molecules and proteins, Universal.