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目的研究广东省广州市5岁以下儿童秋冬季急性病毒性腹泻诺如病毒的感染情况、基因型别和流行优势毒株。方法采用逆转录-聚合酶链反应(RT-PCR)方法检测诺如病毒(NVs),部分阳性标本的PCR产物经纯化、测序,结合参考毒株相应的核苷酸序列进行进化分析。结果NVs的检出率为23.88%(112/469)。35株测序病毒之间的核苷酸序列同源性为70.4%~100%,均属于GII遗传组。广州市2003年秋冬季测序的11株NVs 5株为GII-4;5株为GII-3;另1株不能确定其型别;2004年测序的11株10株为GII-4;1株为GII-3;2005年所测序的13株均为GII-4。结论广州市婴幼儿秋冬季病毒性腹泻存在不同基因型NVs感染,以NV GII组毒株为主,其中GII-4型为流行优势株。
Objective To study the infection status, genotype and prevalence of acute viral diarrhea Norovirus in children under 5 years of age in Guangzhou, Guangdong Province. Methods Norovirus (NVs) were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The PCR products of some positive samples were purified and sequenced, and the nucleotide sequences of the corresponding strains were analyzed for evolution. Results The detection rate of NVs was 23.88% (112/469). The nucleotide sequence homologies between 35 sequenced viruses ranged from 70.4% to 100%, belonging to the GII genetic group. 11 isolates from Guangzhou in the autumn and winter of 2003 were GII-4; 5 isolates were GII-3; the other isolates were not confirmed by their genotypes; 11 isolates were sequenced in 2004 and 10 isolates were GII-4; - 3; all 13 isolates sequenced in 2005 were GII-4. Conclusion There are different genotypes of NVs infection in autumn and winter viral diarrhea in infants and young children in Guangzhou. The genotypes of NV GII were predominant. Among them, GII-4 was the dominant strain.