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目的:了解柯萨奇B组病毒(CVB)在心脏疾病患者中的感染状况,探讨CVB感染与心血管疾病的内在关系。方法:采用间接酶联免疫吸附测定(ELISA)和反转录-聚合酶链反应(RT-PCR),进行CVBIgG抗体和核酸检测。结果:心脏病组CVB IgG阳性率为44.8%(112/250),对照组抗体阳性率为16.7%(18/108),两者有显著性差异(P<0.05)。CVB RNA检测结果显示,心脏病组阳性率为20.4%(51/250),对照组为3.7%(4/108),两者比较有显著性差异(P<0.05)。结论:在心脏病患者中CVB感染较普遍,应引起重视。
Objective: To understand the infection status of Coxsackie B virus (CVB) in patients with heart disease and to explore the relationship between CVB infection and cardiovascular disease. Methods: CVBIgG antibodies and nucleic acids were detected by indirect enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Results: The positive rate of CVB IgG in heart disease group was 44.8% (112/250). The positive rate of antibody in control group was 16.7% (18/108). There was a significant difference between the two groups (P <0.05). The results of CVB RNA showed that the positive rate of heart disease was 20.4% (51/250) and that of the control group was 3.7% (4/108). There was a significant difference between the two groups (P <0.05). Conclusions: CVB infection is common in patients with heart disease and should be emphasized.