论文部分内容阅读
Licochalcone A是新疆甘草的主要活性物质,是从胀果甘草Glycyrrhiza inflata Beta根中得到的。曾报道Licochalcone A于体外具有抗HIV、自由基清除、抗人多核粒细胞白三烯合成及黄嘌呤氧化酶的作用。本文介绍了Lic-ochalcone A的抗炎及抗肿瘤作用。新疆甘草用EtOAc提取后用硅胶层析(流动相CH_3Cl_3-MeOH=99:1),得富含Lic-ochalcone A的部分,再经HPLC制备色谱分离,CH_3CN-3％HOAc=45:55洗脱,以甲醇重结晶得Licochlcone A的黄色针状晶体。 Licochlcone A 0.5rag/耳对12-O-14酰波醇-13-乙酸酯(TPA)及花生四烯酸(AA)渗发的ddy小鼠耳水肿有明显抑制作用。用Lic-ochlcone A与Hela细胞培养1h再与~(32)P_1和
Licochalcone A is the main active substance of Xinjiang licorice, which is obtained from the root of Glycyrrhiza inflata beta licorice. Licochalcone A has been reported to have anti-HIV, free radical scavenging, anti-human leukotriene synthesis and xanthine oxidase in vitro. This article describes the anti-inflammatory and anti-tumor effects of Lic-ochalcone A. Xinjiang licorice was extracted with EtOAc and then chromatographed on silica gel (mobile phase CH 3 Cl 3 -MeOH = 99:1) to obtain a fraction rich in Lic-ochalcone A, which was separated by HPLC preparative chromatography. CH_3CN-3% HOAc=45:55 Elution Recrystallization of methanol gave Licochlcone A as yellow needles. Licochlcone A 0.5rag/ear had significant inhibitory effects on 12-O-14 acyl alcohol-13-acetate (TPA) and arachidonic acid (AA) infiltrated ddy mouse ear edema. Incubation with Lic-ochlcone A and Hela cells for 1 h followed by ~(32)P_1