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目的 :评价用低温营养液保存的羊膜在眼表疾病手术中应用的效果。方法 :对 5 4例 (5 8只眼 )眼表疾病患者施行眼表重建手术 ,其中 34例 38只眼用低温营养液保存的羊膜 ,并与用新鲜羊膜移植的 2 0例 2 0只眼作对照。结果 :新鲜羊膜与营养液保存羊膜在眼表重建中均有良好的作用 ,手术后早期 ,尤其是术后第 1～ 2天新鲜羊膜的上皮完整性略优于营养液保存羊膜 ,但差异无显著性 ,7天后新鲜羊膜与营养液保存羊膜修复情况相同 ;两组角膜上皮均于术后 8天完全修复。两组羊膜移植治疗患者在术后视力恢复、术后反应、羊膜成活率等对照无显著性差异。结论 :低温营养液保存对羊膜损伤小 ,破坏少 ,有利于羊膜活性成分的保存。新鲜和低温营养液保存羊膜均可有效地用于重建眼表缺损 ,对治疗眼表疾病具有安全有效 ,恢复快 ,使用方便等优点
OBJECTIVE: To evaluate the effect of amniotic membrane preserved with hypothermic nutrient solution in ocular surface disease surgery. Methods: Echocardiographic reconstructive surgery was performed on 54 patients (58 eyes) with ocular surface disease. Thirty-four eyes (38 eyes) were preserved amniotic membrane with cryogenic nutrient solution and compared with 20 cases (20 eyes) with fresh amniotic membrane transplantation As a control. RESULTS: Fresh amniotic membrane and nutrient solution preserved amniotic membrane all played a good role in ocular surface reconstruction. The epithelial integrity of fresh amniotic membrane was slightly better than that of nutrient solution in the early postoperative period, especially in the first to the second postoperative days, but the difference was no Significance, fresh amniotic membrane after 7 days and nutrient solution preservation of amniotic membrane repair the same situation; two groups of corneal epithelium were completely repaired after 8 days. The two groups of amniotic membrane transplantation in patients with postoperative visual acuity, postoperative response, survival rate of amniotic membrane and other controls no significant difference. Conclusion: The preservation of hypothermic nutrient solution has less damage to amniotic membrane and less destruction, which is good for the preservation of amniotic active ingredients. Amniotic fluid preservation by both fresh and low-temperature nutrient solution can be effectively used to reconstruct ocular surface defects, which is safe and effective for the treatment of ocular surface diseases, quicker recovery and easy to use