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目的了解三亚市食物中毒中人感染副溶血性弧菌携带毒素基因及耐药性情况,为副溶血性弧菌食物中毒病人的治疗提供参考依据。方法利用19种不同的抗生素药敏纸片,采用K-B扩散法进行耐药检测;用荧光定量PCR扩增法,以检测菌株的TDH和TRH基因。结果经检测的30份菌株,28株携带有TDH基因,阳性率93%,所有菌株均没携带TRH基因。30份菌株对诺氟沙星、头孢曲松、氧氟沙星、头孢哌酮、庆大霉素、氯霉素、头孢克洛、氨苄青霉素、妥布霉素、环丙沙星、洛美沙星、依诺沙星、亚胺培南、萘啶酸未见有耐药株;青霉素G、苯唑西林、羧苄青霉素全部耐药;头孢噻吩、链霉素部分耐药。结论带有TDH毒力基因是三亚市近年来引起食物中毒中人感染副溶血性弧菌主要分子流行病学特征,所分离菌株对大多数常见抗生素敏感性高。研究结果为评价引起食物中毒的副溶性弧菌的分子流行病学特征和制定可行的防控措施提供了科学依据。
Objective To understand the gene and drug resistance of Vibrio parahaemolyticus carrying human toxins in food poisoning in Sanya City and provide a reference for the treatment of patients with Vibrio parahaemolyticus food poisoning. Methods Nineteen different antibiotic susceptibility disks were used to detect drug resistance by K-B diffusion method. Fluorescent quantitative PCR was used to detect the TDH and TRH genes of the strains. Results Tested 30 strains, 28 strains carrying TDH gene, the positive rate of 93%, all strains did not carry TRH gene. 30 strains were tested against norfloxacin, ceftriaxone, ofloxacin, cefoperazone, gentamicin, chloramphenicol, cefaclor, ampicillin, tobramycin, ciprofloxacin, Star, enoxacin, imipenem, nalidixic acid no resistant strains; penicillin G, oxacillin, carbenicillin resistance; cefalothin, streptomycin partially resistant. Conclusion The TDH virulence genes are the main molecular epidemiological characteristics of human Vibrio parahaemolyticus caused by food poisoning in Sanya City in recent years. The isolated strains are highly sensitive to most common antibiotics. The results provide a scientific basis for evaluating the molecular epidemiology of Vibrio parahaemolyticus causing food poisoning and formulating feasible control measures.