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目的采用一测多评法同时测定妇乐颗粒中绿原酸、大黄素、大黄酚与延胡索乙素的含量。方法采用Agilent ZORBAX Eclipse XDB C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈(A)-0.2%磷酸水溶液(B),梯度洗脱:0~8.0 min,85%B;8.0~15.0 min,85%→45%B;15.0~30.0 min,45%→20%B;分段变波长测定:0~8.0 min为327 nm,8.0~15.0 min为254 nm,15.0~30.0 min为280 nm;流速为1.0 m L·min~(-1);柱温为30℃。测定大黄素与绿原酸、大黄酚和延胡索乙素间的相对校正因子,考察其重复性,比较计算值与测得值的差异。结果用一测多评法对6批妇乐颗粒中绿原酸、大黄素、大黄酚与延胡索乙素进行测定,与外标法实测值基本一致。结论该方法可靠,结果准确,可用于妇乐颗粒的质量控制。
Objective To determine the contents of chlorogenic acid, emodin, chrysophanol and tetrahydropalmatine in Fule Granules by a multi-evaluation method. Methods The mobile phase consisted of acetonitrile (A) - 0.2% phosphoric acid solution (B) with gradient elution: 0 ~ 8.0 min, 85% B on an Agilent ZORBAX Eclipse XDB C18 column (4.6 mm × 250 mm, 15.0 min to 30.0 min, 45% to 20% B; the determination of the variable wavelength of section was 327 nm at 0-8.0 min, 254 nm at 8.0-15.0 min and 280 at 15.0-30.0 min nm. The flow rate was 1.0 m L · min -1. The column temperature was 30 ℃. The relative correction factors between emodin and chlorogenic acid, chrysophanol and tetrahydropalmatine were determined and their repeatability was investigated. The differences between calculated and measured values were compared. Results A multi-evaluation method was used to determine the contents of chlorogenic acid, emodin, chrysophanol and tetrahydropalmatine in 6 batches of Fule granules, which were basically in accordance with the measured data of external standard method. Conclusion The method is reliable and accurate. It can be used for the quality control of Fule granules.