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本文通过亲脂毒素PbT_x-3的活体和离体经皮渗透试验旨在 (1) 评价离体试验方法对确定亲脂化合物经皮渗透的意义;(2) 证明真皮作为储存库对局部吸收亲脂化合物的作用。实验动物选用9～17kg的断奶幼猪。涂皮用~3H-PbT_x-3,放射性比度为0.9～1.8Ci/mmol,放化纯度>98％。PbT_x-3的溶剂为二甲基亚砜(DMSO)。活体经皮吸收试验的涂皮剂量为0.4μg/cm~2,流动扩散室离体皮肤渗
In vivo and in vitro transdermal penetration testing of lipophilic toxin PbT_x-3 is intended to (1) evaluate the significance of the in vitro test method for determining transdermal penetration of lipophilic compounds; (2) demonstrate that dermis acts as a reservoir for local absorption pro- The role of lipid compounds. Experimental animals choose 9 ~ 17kg of weaned piglets. Skin coating with ~ 3H-PbT_x-3, radioactivity ratio of 0.9 ~ 1.8Ci / mmol, radiochemical purity> 98%. The solvent for PbT_x-3 is dimethylsulfoxide (DMSO). In vivo percutaneous absorption test dermal dose of 0.4μg / cm ~ 2, flow diffusion chamber ex vivo skin seepage