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目的：了解2013~2014年上饶市乙型流感病毒HA1基因的特性变化情况。方法选取2013年5月~2014年4月年间狗肾传代细胞(MDCK)培养分离到的部分乙型流感毒株,提取病毒RNA,采用逆转录和聚合酶链式反应扩增乙型流感病毒血凝素基因,扩增产物经纯化后,进行血凝素基因HA1区和核苷酸序列测定并推导出氨基酸序列,采用DNAStar5.0和MEGA4.0生物软件对测序结果进行基因特性分析。结果2013~2014年间上饶市人群中流行的乙型流感病毒均为Yamagata亚型,通过对测序的序列分析,我市的流行株与推荐的疫苗株B/Mas achuset s/02/2012进行比较发现,氨基酸变异数较高为11~14个,其中有4个变异分别分布在其抗原决定簇120环,160环和190环中。结论2013~2014年上饶市人群中流行的乙型Yamagata亚型流感病毒基因特性发生了一些改变,导致本市仍发生了乙型Yamagata亚型流感病毒的流行。“,”Objective To characterize HA1gene of influenza B virus circulated in 2013 through 2014 in Shangrao city.Methods Influenza B virus were isolated with MDCK cel s during May2013-April2014,extracted RNA.HA genes of influenza B viruses were amplifide by RT-PCR and virion RNA products were purified and nucleotied sequences of HA1 gene were analyzed and deduced to determine the ino acid sequences.Final y the characterization was analyzed by DNAStar5.0 and MEGA4.0.Results Yamagata was only popular B flu viruse during 2013~2014 in Shangrao.Analyzed by software that compared with B/Massachuset s/02/2012,the viruses had 11~14 am ino acids substitution,including four variation were distributed in its epitopes 120 ring,160 ring and 190 ring. Conclusion The genetic characteristics of influenza B viruses had changed during 2013~2014 in Shangrao,so when the waccine strains can not protect them,leading to the city stil occured subtype of influenza B Yamagata virus epidemic.