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目的 设计出在体外检测联苯双酯作用效果的实验,以观测该药是否为转氨酶的可逆性抑制物。方法 应用26份血清,混合离心分装每份400μl,分别用生理盐水和1500 μl/ml的联苯双酯溶液稀释进行谷-丙转氨酶(ALT)和谷-草转氨酶(AST)测定。结果 将结果进行直线回归分析,发现用药组之AST和ALT活性比盐水组略高。结论 本实验表明转氨酶的活性并不受联苯双酯溶液浓度的影响,联苯双酯可能不是转氨酶的可逆性抑制物。
Objective To design an experiment to test the effect of bifendate in vitro in order to observe whether the drug is a reversible inhibitor of transaminase. Methods Serum ALT and AST were determined by diluting 26 serum samples into 400μl aliquots mixed with normal saline and 1500μl / ml bifendate respectively. Results The results were linear regression analysis and found that the treatment group of AST and ALT activity slightly higher than the saline group. Conclusion This experiment shows that transaminase activity is not affected by the concentration of bifendate solution. Bifendate may not be a reversible inhibitor of transaminase.