我们进行了脉动真空蒸汽灭菌柜的确认及验证工作,以葡萄糖肉汤培养基为例,我们进行了热穿透,热分布的数据分析,生物挑战实验确认,和培养基促生长实验确认,针对本公司脉动真空灭菌柜特定型号液体装载,我们设定的灭菌参数既能达到药典的无菌要求,又能保证培养基的促生长实验要求。 We carried out the confirmation and validation of pulsating vacuum steam sterilizers. Taking glucose broth as an example, we conducted data analysis of heat penetration and heat distribution, confirmed the biological challenge experiment, For the specific type of liquid loading of our pulsation vacuum sterilizer, the sterilization parameters we set can not only meet the sterility requirements of Pharmacopoeia, but also ensure the growth-promoting requirements of the medium.