论文部分内容阅读
目的:研究并回顾性分析喉鳞状细胞癌(LSCC)及癌旁组织中p53基因的表达及其与生物学行为的关系和意义。方法:用免疫组织化学SP法检测76例LSCC及癌旁组织中的p53表达。结果:LSCC组织中p53过表达47.4%(36/76),对应原位癌p53过表达为40.0%(4/10);癌旁非典型增生组织中p53过表达22.2%(8/36);癌旁正常组织中p53无过表达(0/56);p53在LSCC实质及相应原位癌中的表达呈现完全一致性。p53在癌旁非典型增生组织和对应癌实质组织中的过表达呈现高度显著正相关性(P<0.01);p53在癌组织中的过表达显著高于非典型增生组织(P<0.01);p53过表达和性别、年龄、临床分期、临床LSCC类型(LSCC位置)、肿瘤大小均无统计学意义,和病理分级(G1→G2+G3)有高度显著相关性(P<0.01)。p53在LSCC淋巴结转移组的过表达(68.8%)高于非转移组中的过表达(41.7%),但差异无统计学意义。LSCC病理分级(G1→G2+G3)和淋巴结转移有高度显著相关性(P<0.01)。结论:p53异常表达可出现于LSCC不同阶段及癌旁癌前病变组织中,并具有显著相关性,是LSCC发生、发展的重要因素及有意义的早期改变,并可反映喉癌组织细胞增殖活性和恶性程度及预后。
Objective: To investigate and retrospectively analyze the relationship between p53 gene expression and biological behavior in laryngeal squamous cell carcinoma (LSCC) and its adjacent tissues. Methods: The expression of p53 in 76 cases of LSCC and its adjacent tissues was detected by immunohistochemical SP method. Results: The overexpression of p53 in LSCC was 47.4% (36/76), corresponding to the overexpression of p53 in situ (40.0%, 4/10). The overexpression of p53 in the adjacent non - cancerous tissues was 22.2% (8/36). There was no overexpression of p53 in adjacent normal tissues (0/56). The expression of p53 in LSCC and corresponding carcinoma in situ was completely consistent. There was a significant positive correlation between the expression of p53 and adjacent cancerous tissues (P <0.01). The overexpression of p53 was significantly higher in atypical hyperplastic tissues than in atypical hyperplastic tissues (P <0.01). There was no significant difference in the expression of p53, gender, age, clinical stage, clinical LSCC type (LSCC location), and tumor size (P <0.01). The overexpression of p53 (68.8%) in LSCC lymph node metastasis group was higher than that in non-metastasis group (41.7%), but the difference was not statistically significant. There was a highly significant correlation between LSCC pathological grade (G1 → G2 + G3) and lymph node metastasis (P <0.01). Conclusion: Aberrant expression of p53 may occur in different stages of LSCC and precancerous lesions, with significant correlation. It is an important factor for the occurrence and development of LSCC and a meaningful early change. It can also reflect the cell proliferation activity of LSCC And the degree of malignancy and prognosis.