论文部分内容阅读
目的:探讨黄芪注射液对H2O2损伤L6大鼠成肌细胞的修复。方法:取1、0.5、0.1、0.05mmol/L H2O2损伤成肌细胞建立模型,再取2 000、1 000、500、250、125、62.5mg/mL黄芪注射液实施保护。应用MTT法、流式细胞仪及荧光抗体Bax和Bcl-2检测,使用SPSS 15.0统计软件处理数据。结果:经0.1mmol/L H2O2损伤成肌细胞存活率由94.4±5.7%降至32.6±3.8%,凋亡率由0增至32.45±2.9%。黄芪注射液处理实验组细胞存活率64.5±4.8～82.2±9.6%较模型组32.6±3.8%增加,凋亡率也从32.45±2.9%减至2.96±0.5～9.29±2.7%,荧光抗体检测Bcl-2细胞数57.7±4.3～74.2±6.9,Bax细胞数42.3±4.0～60.2±5.1。结论:黄芪注射液对大鼠成肌细胞H2O2损伤经由p38MAPK信号通路实施保护。
Objective: To investigate the effects of Astragalus membranaceus injection on myoblast repair in H2O2 injured L6 rats. Methods: The models of injured myoblasts were established with 1, 0.5, 0.1, 0.05 mmol / L H2O2, and then were protected with 2 000, 1 000, 500, 250, 125 and 62.5 mg / mL Astragalus injection. MTT assay, flow cytometry and fluorescent antibody Bax and Bcl-2 detection, the use of SPSS 15.0 statistical software processing data. Results: The viability of myoblasts injured by 0.1 mmol / L H2O2 decreased from 94.4 ± 5.7% to 32.6 ± 3.8%, and the apoptosis rate increased from 0 to 32.45 ± 2.9%. Astragalus injection treatment experimental group cell survival rate of 64.5 ± 4.8 ~ 82.2 ± 9.6% compared with the model group 32.6 ± 3.8% increase in apoptosis rate from 32.45 ± 2.9% to 2.96 ± 0.5 ~ 9.29 ± 2.7%, fluorescent antibody detection of Bcl -2 cells was 57.7 ± 4.3 ~ 74.2 ± 6.9, and the number of Bax cells was 42.3 ± 4.0 ~ 60.2 ± 5.1. Conclusion: Astragalus injection on rat myoblasts H2O2 injury by p38 MAPK signaling pathway protection.