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目的比较降压避风片新老配方的降压效果及作用特点。方法选用原发性高血压大鼠(SHR)及其对照性WKY大鼠进行对比试验。实验动物分为8组:WKY大鼠组、SHR对照组、降压避风片老配方组高、中、低剂量(1.00、0.50、0.25 g药粉/kg)组、降压避风片新配方高、中、低剂量(1.00、0.50、0.25 g药粉/kg)组。降压试验采用大鼠无创血压测定仪对大鼠尾动脉进行血压测定,分为急性降压作用试验和慢性降压验。急性降压测定给药前、给药后1、3 h血压值;慢性降压测定连续给药后6、11、14 d血压值。连续给药14 d后,进行大鼠心脏超声心动试验,监测大鼠室间隔厚度、左室内径、左室后壁、射血分数、左室短轴缩短率、左心室心肌质量、左心室容量等;后腹主动脉取血,分别测定血清NO、总蛋白、白蛋白、球蛋白、丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、尿素、肌酐、葡萄糖、三酰甘油、低密度脂蛋白和血浆内皮素、肾素活性及血管紧张素Ⅱ含量。结果急性降压试验:各剂量组均可显著降低药后3 h的收缩压(P<0.01),新老配方相应剂量间无显著差异;慢性降压试验:SHR组心率显著快于WKY组,用药后,各剂量组均无显著降低心率的效果。结论新老配方均可平稳降低SHR血压,降压作用确切。
Objective To compare the antihypertensive effects and the action characteristics of antihypertensive and antihypertensive tablets. Methods Primary hypertensive rats (SHR) and its control WKY rats were compared. The experimental animals were divided into 8 groups: WKY rats, SHR control group, high, medium and low dose (1.00, 0.50, 0.25 g powder / kg) Medium and low dose (1.00,0.50,0.25 g powder / kg) group. Antihypertensive test rat noninvasive blood pressure meter rat tail artery blood pressure measurement, divided into acute antihypertensive effect test and chronic hypotensive experience. Acute antihypertensive before and after administration, 1,3 h after administration of blood pressure values; chronic antihypertensive determination of continuous administration of blood pressure after 6,11,14 d. After continuous administration for 14 days, rat heart echocardiography was performed to monitor the interventricular septum thickness, left ventricular diameter, left ventricular posterior wall, ejection fraction, shortening of left ventricular short axis, left ventricular myocardial mass, left ventricular volume The blood samples were taken from the abdominal aorta and measured for serum NO, total protein, albumin, globulin, alanine aminotransferase, aspartate aminotransferase, urea, creatinine, glucose, triglyceride, Lipoprotein and plasma endothelin, renin activity and angiotensin Ⅱ content. Results Acute antihypertensive test: The systolic blood pressure at 3 h post-treatment was significantly decreased in each dose group (P <0.01), and no significant difference was found between the corresponding dose of new-old formula. Chronic hypotensive test: heart rate in SHR group was significantly faster than that in WKY group After treatment, each dose group did not significantly reduce the effect of heart rate. Conclusion Both new and old prescriptions can steadily reduce the blood pressure of SHR, and the antihypertensive effect is definite.