目的:研究补肾中药复方与跑台训练联合干预对模拟失重大鼠骨密度和骨代谢指标的影响。方法:9周龄Wistar雄性大鼠40只,随机分为对照组、失重对照组、中药干预组、跑台训练干预组、中药+跑台训练联合干预组5组,每组8只。采用后肢悬吊方法制备模拟失重大鼠模型,悬吊4周。悬吊结束后,分别给予补肾中药复方、跑台训练及中药+跑台训练联合干预措施,干预周期均为8周。8周后,测试各组大鼠左侧股骨骨密度和血清骨代谢指标血钙、碱性磷酸酶、骨钙素和抗酒石酸酸性磷酸酶5b。结果:失重对照组大鼠血钙和抗酒石酸酸性磷酸酶5b显著高于正常对照组(P<0.01),而血清碱性磷酸酶和骨钙素差异无统计学意义(P>0.05),骨密度显著低于正常对照组(P<0.01)。中药干预组大鼠血钙和抗酒石酸酸性磷酸酶5b显著低于失重对照组(P<0.05,P<0.01),而血清碱性磷酸酶和骨钙素差异无统计学意义(P>0.05),骨密度显著高于失重对照组(P<0.01)。跑台干预组大鼠血钙和抗酒石酸酸性磷酸酶5b显著低于失重对照组(P<0.05,P<0.01),血清碱性磷酸酶和骨钙素显著高于失重对照组(P<0.01),骨密度显著高于失重对照组(P<0.01)。中药+跑台训练联合干预组大鼠血钙和抗酒石酸酸性磷酸酶5b显著低于失重对照组(P<0.01),血清碱性磷酸酶和骨钙素显著高于失重对照组(P<0.01)。联合干预组血钙和抗酒石酸酸性磷酸酶5b显著低于中药干预组和跑台训练干预组(P<0.05,P<0.01),而血清碱性磷酸酶和骨钙素与各单一干预组比较差异无统计学意义,骨密度显著高于中药干预组和跑台训练干预组(P<0.01)。结论:补肾中药复方和跑台训练联合干预有效抑制模拟失重大鼠骨吸收,增强骨形成,升高骨密度,其效果优于单纯中药复方或跑台训练干预。 OBJECTIVE: To study the effects of combined TCM of tonifying kidney prescription and treadmill training on bone mineral density and bone metabolism in simulated weightless rats. Methods: Forty Wistar male rats aged 9 weeks were randomly divided into control group, weightless control group, traditional Chinese medicine intervention group, treadmill training intervention group, traditional Chinese medicine + treadmill training intervention group, with 8 rats in each group. The hindlimb suspension method was used to prepare the simulated weightless rat model and was suspended for 4 weeks. After the suspension, they were given kidney Chinese compound, treadmill training and traditional Chinese medicine + treadmill training intervention intervention, the intervention cycle were 8 weeks. After 8 weeks, the left femur BMD and serum BMI were tested in each group. Serum calcium, alkaline phosphatase, osteocalcin and tartrate-resistant acid phosphatase 5b were measured. Results: The levels of serum calcium and tartrate-resistant acid phosphatase 5b in control group were significantly higher than those in normal control group (P <0.01), while there was no significant difference in serum alkaline phosphatase and osteocalcin (P> 0.05) The density was significantly lower than the normal control group (P <0.01). The levels of serum calcium and tartrate-resistant acid phosphatase 5b in Chinese medicine intervention group were significantly lower than those of weightless control group (P <0.05, P <0.01), while there was no significant difference in serum alkaline phosphatase and osteocalcin (P> 0.05) , Bone mineral density was significantly higher than the weight loss control group (P <0.01). Serum calcium and tartrate-resistant acid phosphatase 5b were significantly lower in treadmill intervention group than in weightless control group (P <0.05, P <0.01), serum alkaline phosphatase and osteocalcin were significantly higher than those in control group (P <0.01) ), Bone mineral density was significantly higher than that of the control group (P <0.01). The levels of serum calcium and tartrate-resistant acid phosphatase 5b in Chinese medicine + treadmill training intervention group were significantly lower than those in weightless control group (P <0.01), serum alkaline phosphatase and osteocalcin were significantly higher than those in control group (P <0.01) ). The levels of serum calcium and tartrate-resistant acid phosphatase 5b in the combined intervention group were significantly lower than those in the traditional Chinese medicine intervention group and the treadmill training intervention group (P <0.05, P <0.01), while serum alkaline phosphatase and osteocalcin were significantly lower than those in the single intervention group The difference was not statistically significant. The bone mineral density was significantly higher than that of traditional Chinese medicine intervention group and treadmill training intervention group (P <0.01). Conclusion: The combination of tonifying traditional Chinese medicine and treadmill training can effectively inhibit bone resorption, enhance bone formation and increase bone density in simulated weightless rats, which is better than that of traditional Chinese medicine or treadmill training.