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目的:研究西达本胺对前列腺癌DU145、PC3细胞生长抑制及凋亡调控的作用。方法:设置西达本胺浓度分别为4、8、16、32和64μmol/L的5个处理组和对照组(未加西达本胺),分别处理DU145、PC3细胞不同时间,采用MTT法检测细胞的增殖抑制情况,用倒置显微镜观察细胞形态,用流式细胞仪进行细胞凋亡分析,用免疫印迹法检测细胞内Bax、Bcl-2、caspase-3、caspase-9的表达水平。结果:与对照组比较,西达本胺处理组可使细胞明显变圆、体积缩小、脱壁细胞增多;MTT法检测显示,随着西达本胺浓度的增加和作用时间的延长,对DU145、PC3细胞的增殖抑制作用增强,并呈时间、剂量正相关(P<0.01);流式细胞术显示,对照组和16、64μmol/L西达本胺组细胞凋亡率分别为42.24%、50.23%;随着西达本胺浓度的增加,Bcl-2的表达呈下调趋势,Bax、caspase-3、caspase-9的表达呈上调趋势,呈剂量正相关。结论:西达本胺对前列腺癌DU145、PC3细胞生长具有明显的抑制作用,作用机制可能与Bax、Bcl-2、caspase-3及caspase-9凋亡信号通路有关。
OBJECTIVE: To study the effects of crudamine on the growth inhibition and apoptosis of prostate cancer DU145 and PC3 cells. METHODS: Five treatment groups (4, 8, 16, 32 and 64 μmol / L) with and without daidzein were established. The DU145 and PC3 cells were treated with different concentrations of MTT assay The cell proliferation was observed by inverted microscope. Cell apoptosis was analyzed by flow cytometry. The expression of Bax, Bcl-2, caspase-3 and caspase-9 were detected by Western blotting. Results: Compared with the control group, the group treated with coda amine significantly decreased the number of cells and the number of detached cells. The MTT assay showed that with the increase of the concentration of cedilamine and the prolongation of the action time, (P <0.01). The results of flow cytometry showed that the apoptotic rates of control group and 16,64μmol / L cydamine group were 42.24% 50.23%. With the increase of cedilamine concentration, the expression of Bcl-2 was down-regulated. The expressions of Bax, caspase-3 and caspase-9 were up-regulated. CONCLUSION: Cidabain can significantly inhibit the growth of prostate cancer cells DU145 and PC3, and the mechanism may be related to the signal pathways of apoptosis of Bax, Bcl-2, caspase-3 and caspase-9.