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目的:对急性阑尾炎患者手术后炎症组织进行细菌学培养,分析其病原菌分布及耐药性,指导临床抗菌药物应用。方法:采用VTTEK-32全自动细菌鉴定系统,对180例急性阑尾炎患者行阑尾切除术后,对炎症组织标本做细菌培养及药敏试验。结果:180例标本中有134例检出细菌,阳性率74.4%,分离到细菌140株,其中革兰阴性细菌102株,革兰阳性细菌38株。主要感染细菌为大肠埃希菌、铜绿假单胞菌等。药敏试验结果主要是对头孢匹林、亚培胺南等敏感。结论:急性阑尾炎患者感染细菌以肠杆菌为主,其对第三代头孢类较敏感,临床用药时应予以重视。
Objective: To study the bacteriological culture of inflammatory tissues in patients with acute appendicitis after surgery, analyze the distribution and drug resistance of pathogens, and guide the clinical application of antibacterial drugs. Methods: VTTEK-32 automatic bacterial identification system was used in 180 cases of acute appendicitis patients underwent appendectomy, the inflammatory tissue specimens for bacterial culture and drug susceptibility testing. Results: Of the 180 specimens, 134 cases were detected, the positive rate was 74.4%, and 140 strains were isolated, of which 102 were Gram-negative bacteria and 38 were Gram-positive bacteria. The main bacterial infection is Escherichia coli, Pseudomonas aeruginosa and so on. Susceptibility test results are mainly sensitive to cefpirome, abamelamine and other South. Conclusion: Infectious bacteria in patients with acute appendicitis are mainly Enterobacteriaceae, which are more sensitive to the third generation of cephalosporins and should be taken seriously in clinical application.